Analysis Note

Positive ControlHep G2 or NIH 3T3 cells

Application

Frozen Sections (5-10 µg/ml)r>Immunoblotting (1-5 µg/ml)r>Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1-2 µg per reaction)r>Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)r>Gel Shift (see comments and application references)r>Free-floating Sections (not recommended)

General description

This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen Sections, WB, IF, IP, Paraffin Sections, Gel Shift, for the detection of c-Fos (Ab-2) (4-17).

The proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.

Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.

Immunogen

a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.

DeTogni, P., et al. 1988. Cell Biol.8, 2251.Rouscher III, F.J., et al. 1988. Science240, 1010.Sassone-Corsi, P., et al. 1988. Cell54, 553.Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.Muller, R. 1986. Biochim. Biophys. Acta823, 207.Verma, I. 1986. Trends Genet.2, 93.Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433

Packaging

100 µg in Glass bottle

Physical form

Lyophilized from PBS, a volatile buffer, 100 µg BSA.

Reconstitution

Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.

Warning

Toxicity: Standard Handling (A)